![]() Removal of host DNA increases the coverage of microbial reads in sequencing experiments. Thus, maximized coverage of microbial reads greatly enhances analysis power. ![]() In contrast to 16S rDNA sequencing, whole metagenome shotgun sequencing can add valuable insight to microbiome studies, such as the presence of virulence factors, antibiotic resistance, or metabolic networks. In fact, a key finding of the whole metagenome shotgun sequencing of different sample types conducted by the Human Microbiome Project was that up to 99% of the sequencing reads corresponded to the human genome, so that at most 1% were of microbial nature. In metagenomic studies, total DNA is standardly isolated and analyzed, however, host human or animal DNA greatly outweighs the microbial DNA, which can hamper microbiome analysis. Swabs and body fluids contain DNA from host human or animal cells as well as microbial cells. Effective host DNA depletion for deeper microbiome analysis
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